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[Case study] CRISPR library screen for target candidates -- Essential genes in AGS cell line Previously we released an article for CRISPR library, which mentioned the application of CRISPR library (Click here to review CRISPR library 101 (PartI) what are the popular applications o…
Utilize CRISPR/Cas9 wisely to study kinase, explore the inner mechanism of biological activities Modern biology take s protein as the basis of all cells and organisms, which is the main undertaker of life activities. However, if you want to make life activities regular ly, you mus…
[Research highlight] CRISPR-B TM knockout E.coli facilitates MMR sliding clamp research DNA mismatch repair (MMR) is a repair system after genome replication, which plays an important role in improving DNA replication fidelity, reducing gene mutation and maintaining genome stabili…
Having trouble with cell transfection? Did you pick the right method? Transfection is a very common operation in cell experiments. Through transfection, foreign nucleic acids (DNA, RNA, etc.) can be introduced into eukaryotic cells to study the function of genes, which is an essen…
CRISPR/Cas9 facilitate to discover of inhibitors of KRAS, the difficult-to-target gene in cancer KRAS mutation often occurs in various typesof tumors. About 30% of cancer patients have KRAS gene mutations in their genomes, including 90% ofpancreatic cancer, 50% of colon cancer and…