ABCB4 Knockout cell line (IPSC)

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ABCB4 Knockout cell line (IPSC) Order

ABCB4 Knockout cell line (IPSC)
Catalog#
YKO-H1186
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ABCB4 Knockout cell line (IPSC)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ABCB4 Knockout cell line (IPSC)
Species
Human
Gene Name
ABCB4
Gene ID
5244
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript ABCB4-212)
Validation: PCR+Sanger Sequencing
ABCB4 Knockout cell line (IPSC) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ABCB4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the IPSC cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ABCB4 gene knockout ABCB4 Knockout cell line (IPSC) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ABCB4 Knockout cell line (IPSC)
ABCB4 Knockout cell line (IPSC)
Catalog#
YKO-H1186
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

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