AKR7L Knockout cell line (Hep G2)

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AKR7L Knockout cell line (Hep G2) Order

AKR7L Knockout cell line (Hep G2)
Catalog#
YKO-HT7393
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AKR7L Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
AKR7L Knockout cell line (Hep G2)
Species
Human
Gene Name
AKR7L
Gene ID
246181
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript AKR7L-201)
Validation: PCR+Sanger Sequencing
AKR7L Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AKR7L gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AKR7L gene knockout AKR7L Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
AKR7L Knockout cell line (Hep G2)
AKR7L Knockout cell line (Hep G2)
Catalog#
YKO-HT7393
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • AKR7L Knockout cell line (HeLa)

    AKR7L Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75668
    YKO-XN75668(HeLa)
  • AKR7L Knockout cell line (HCT 116)

    AKR7L Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75667
    YKO-XN75667(HCT 116)
  • AKR7L Knockout cell line (HEK293)

    AKR7L Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75666
    YKO-XN75666(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • AKR7L Knockout cell line (HeLa)

    AKR7L Knockout cell line (HeLa)

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75668
      inquiry   More details
  • AKR7L Knockout cell line (HCT 116)

    AKR7L Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75667
      inquiry   More details
  • AKR7L Knockout cell line (HEK293)

    AKR7L Knockout cell line (HEK293)

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75666
      inquiry   More details

AKR7L Knockout cell line (Hep G2) Order

AKR7L Knockout cell line (Hep G2)
Catalog#
YKO-HT7393
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AKR7L Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
AKR7L Knockout cell line (Hep G2)
Species
Human
Gene Name
AKR7L
Gene ID
246181
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript AKR7L-201)
Validation: PCR+Sanger Sequencing
AKR7L Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AKR7L gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AKR7L gene knockout AKR7L Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
AKR7L Knockout cell line (Hep G2)
AKR7L Knockout cell line (Hep G2)
Catalog#
YKO-HT7393
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • AKR7L Knockout cell line (HeLa)

    AKR7L Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75668
    YKO-XN75668(HeLa)
  • AKR7L Knockout cell line (HCT 116)

    AKR7L Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75667
    YKO-XN75667(HCT 116)
  • AKR7L Knockout cell line (HEK293)

    AKR7L Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75666
    YKO-XN75666(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • AKR7L Knockout cell line (HeLa)

    AKR7L Knockout cell line (HeLa)

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75668
      inquiry   More details
  • AKR7L Knockout cell line (HCT 116)

    AKR7L Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75667
      inquiry   More details
  • AKR7L Knockout cell line (HEK293)

    AKR7L Knockout cell line (HEK293)

    Organism: Human
    Gene Name: AKR7L
    Gene ID: 246181
    Size: 1*10^6
    Catalog#: YKO-XN75666
      inquiry   More details

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