BRCC3 Knockout cell line (Hep G2)

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BRCC3 Knockout cell line (Hep G2) Order

BRCC3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7264
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BRCC3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
BRCC3 Knockout cell line (Hep G2)
Species
Human
Gene Name
BRCC3
Gene ID
79184
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript BRCC3-201)
Validation: PCR+Sanger Sequencing
BRCC3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BRCC3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BRCC3 gene knockout BRCC3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
BRCC3 Knockout cell line (Hep G2)
BRCC3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7264
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Organism: Human
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  • BRCC3 Knockout cell line (HCT 116)

    BRCC3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: BRCC3
    Gene ID: 79184
    Size: 1*10^6
    Catalog#: YKO-XN85965
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    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • BRCC3 Knockout cell line (THP-1)

    BRCC3 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: BRCC3
    Gene ID: 79184
    Size: 1*10^6
    Catalog#: YKO-HT0791
  • BRCC3 Knockout cell line (HeLa)

    BRCC3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: BRCC3
    Gene ID: 79184
    Size: 1*10^6
    Catalog#: YKO-XN85966
  • BRCC3 Knockout cell line (HCT 116)

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    Organism: Human
    Gene Name: BRCC3
    Gene ID: 79184
    Size: 1*10^6
    Catalog#: YKO-XN85965

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