C5orf22 Knockout cell line (Hep G2)

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C5orf22 Knockout cell line (Hep G2) Order

C5orf22 Knockout cell line (Hep G2)
Catalog#
YKO-HT7685
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
C5orf22 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
C5orf22 Knockout cell line (Hep G2)
Species
Human
Gene Name
C5orf22
Gene ID
55322
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript C5orf22-201)
Validation: PCR+Sanger Sequencing
C5orf22 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the C5orf22 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, C5orf22 gene knockout C5orf22 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
C5orf22 Knockout cell line (Hep G2)
C5orf22 Knockout cell line (Hep G2)
Catalog#
YKO-HT7685
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • C5orf22 Knockout cell line (HeLa)

    C5orf22 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86131
    YKO-XN86131(HeLa)
  • C5orf22 Knockout cell line (HCT 116)

    C5orf22 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86130
    YKO-XN86130(HCT 116)
  • C5orf22 Knockout cell line (HEK293)

    C5orf22 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86129
    YKO-XN86129(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • C5orf22 Knockout cell line (HeLa)

    C5orf22 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86131
      inquiry   More details
  • C5orf22 Knockout cell line (HCT 116)

    C5orf22 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86130
      inquiry   More details
  • C5orf22 Knockout cell line (HEK293)

    C5orf22 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86129
      inquiry   More details

C5orf22 Knockout cell line (Hep G2) Order

C5orf22 Knockout cell line (Hep G2)
Catalog#
YKO-HT7685
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
C5orf22 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
C5orf22 Knockout cell line (Hep G2)
Species
Human
Gene Name
C5orf22
Gene ID
55322
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript C5orf22-201)
Validation: PCR+Sanger Sequencing
C5orf22 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the C5orf22 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, C5orf22 gene knockout C5orf22 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
C5orf22 Knockout cell line (Hep G2)
C5orf22 Knockout cell line (Hep G2)
Catalog#
YKO-HT7685
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • C5orf22 Knockout cell line (HeLa)

    C5orf22 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86131
    YKO-XN86131(HeLa)
  • C5orf22 Knockout cell line (HCT 116)

    C5orf22 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86130
    YKO-XN86130(HCT 116)
  • C5orf22 Knockout cell line (HEK293)

    C5orf22 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86129
    YKO-XN86129(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • C5orf22 Knockout cell line (HeLa)

    C5orf22 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86131
      inquiry   More details
  • C5orf22 Knockout cell line (HCT 116)

    C5orf22 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86130
      inquiry   More details
  • C5orf22 Knockout cell line (HEK293)

    C5orf22 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: C5orf22
    Gene ID: 55322
    Size: 1*10^6
    Catalog#: YKO-XN86129
      inquiry   More details

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