CCNF Knockout cell line (Hep G2)

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CCNF Knockout cell line (Hep G2) Order

CCNF Knockout cell line (Hep G2)
Catalog#
YKO-HT7271
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CCNF Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
CCNF Knockout cell line (Hep G2)
Species
Human
Gene Name
CCNF
Gene ID
899
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript CCNF-202)
Validation: PCR+Sanger Sequencing
CCNF Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CCNF gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CCNF gene knockout CCNF Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CCNF Knockout cell line (Hep G2)
CCNF Knockout cell line (Hep G2)
Catalog#
YKO-HT7271
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • CCNF Knockout cell line (HEK293)

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    Gene Name: CCNF
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    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • CCNF Knockout cell line (HeLa)

    CCNF Knockout cell line (HeLa)

    Organism: Human
    Gene Name: CCNF
    Gene ID: 899
    Size: 1*10^6
    Catalog#: YKO-XN22252
  • CCNF Knockout cell line (HCT 116)

    CCNF Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: CCNF
    Gene ID: 899
    Size: 1*10^6
    Catalog#: YKO-XN22251
  • CCNF Knockout cell line (HEK293)

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    Organism: Human
    Gene Name: CCNF
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    Size: 1*10^6
    Catalog#: YKO-XN22250

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