KIF15 Knockout cell line (Hep G2)

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location： Home > Products > KO Cell Lines > KIF15 Knockout cell line (Hep G2)
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KIF15 Knockout cell line (Hep G2) Order

KIF15 Knockout cell line (Hep G2)
Catalog#
YKO-HT7281
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
KIF15 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
KIF15 Knockout cell line (Hep G2)
Species
Human
Gene Name
KIF15
Gene ID
56992
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 11 (based on transcript KIF15-201)
Validation: PCR+Sanger Sequencing
KIF15 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KIF15 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KIF15 gene knockout KIF15 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
KIF15 Knockout cell line (Hep G2)
KIF15 Knockout cell line (Hep G2)
Catalog#
YKO-HT7281
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • KIF15 Knockout cell line (HeLa)

    KIF15 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: KIF15
    Gene ID: 56992
    Size: 1*10^6
    Catalog#: YKO-XN89767
      inquiry   More details
  • KIF15 Knockout cell line (HCT 116)

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    Gene Name: KIF15
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    Size: 1*10^6
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