LANCL1 Knockout cell line (Hep G2)

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LANCL1 Knockout cell line (Hep G2) Order

LANCL1 Knockout cell line (Hep G2)
Catalog#
YKO-HT7284
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LANCL1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
LANCL1 Knockout cell line (Hep G2)
Species
Human
Gene Name
LANCL1
Gene ID
10314
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript LANCL1-207)
Validation: PCR+Sanger Sequencing
LANCL1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LANCL1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LANCL1 gene knockout LANCL1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LANCL1 Knockout cell line (Hep G2)
LANCL1 Knockout cell line (Hep G2)
Catalog#
YKO-HT7284
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • LANCL1 Knockout cell line (HeLa)

    LANCL1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: LANCL1
    Gene ID: 10314
    Size: 1*10^6
    Catalog#: YKO-XN43764
  • LANCL1 Knockout cell line (HCT 116)

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    Organism: Human
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