MPST Knockout cell line (Hep G2)

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location： Home > Products > KO Cell Lines > MPST Knockout cell line (Hep G2)

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MPST Knockout cell line (Hep G2) Order

MPST Knockout cell line (Hep G2)
Catalog#
YKO-HT7513
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MPST Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MPST Knockout cell line (Hep G2)
Species
Human
Gene Name
MPST
Gene ID
4357
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript MPST-206)
Validation: PCR+Sanger Sequencing
MPST Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MPST gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MPST gene knockout MPST Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MPST Knockout cell line (Hep G2)
MPST Knockout cell line (Hep G2)
Catalog#
YKO-HT7513
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

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  • MPST Knockout cell line (HeLa)

    MPST Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09508
    YKO-HS09508(HeLa)
  • MPST Knockout cell line (HCT 116)

    MPST Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09507
    YKO-HS09507(HCT 116)
  • MPST Knockout cell line (HEK293)

    MPST Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09506
    YKO-HS09506(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • MPST Knockout cell line (HeLa)

    MPST Knockout cell line (HeLa)

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09508
      inquiry   More details
  • MPST Knockout cell line (HCT 116)

    MPST Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09507
      inquiry   More details
  • MPST Knockout cell line (HEK293)

    MPST Knockout cell line (HEK293)

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09506
      inquiry   More details

MPST Knockout cell line (Hep G2) Order

MPST Knockout cell line (Hep G2)
Catalog#
YKO-HT7513
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MPST Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MPST Knockout cell line (Hep G2)
Species
Human
Gene Name
MPST
Gene ID
4357
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript MPST-206)
Validation: PCR+Sanger Sequencing
MPST Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MPST gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MPST gene knockout MPST Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MPST Knockout cell line (Hep G2)
MPST Knockout cell line (Hep G2)
Catalog#
YKO-HT7513
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • MPST Knockout cell line (HeLa)

    MPST Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09508
    YKO-HS09508(HeLa)
  • MPST Knockout cell line (HCT 116)

    MPST Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09507
    YKO-HS09507(HCT 116)
  • MPST Knockout cell line (HEK293)

    MPST Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09506
    YKO-HS09506(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • MPST Knockout cell line (HeLa)

    MPST Knockout cell line (HeLa)

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09508
      inquiry   More details
  • MPST Knockout cell line (HCT 116)

    MPST Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09507
      inquiry   More details
  • MPST Knockout cell line (HEK293)

    MPST Knockout cell line (HEK293)

    Organism: Human
    Gene Name: MPST
    Gene ID: 4357
    Size: 1*10^6
    Catalog#: YKO-HS09506
      inquiry   More details

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