MRPL3 Knockout cell line (Hep G2)

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MRPL3 Knockout cell line (Hep G2) Order

MRPL3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7270
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MRPL3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
MRPL3 Knockout cell line (Hep G2)
Species
Human
Gene Name
MRPL3
Gene ID
11222
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript MRPL3-201)
Validation: PCR+Sanger Sequencing
MRPL3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MRPL3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MRPL3 gene knockout MRPL3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MRPL3 Knockout cell line (Hep G2)
MRPL3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7270
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Instruction: EZ-cryo™ Cell Freezing Medium

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  • MRPL3 Knockout cell line (HeLa)

    MRPL3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: MRPL3
    Gene ID: 11222
    Size: 1*10^6
    Catalog#: YKO-XN45784
  • MRPL3 Knockout cell line (HCT 116)

    MRPL3 Knockout cell line (HCT 116)

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    Gene Name: MRPL3
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