OOSP4A Knockout cell line (Hep G2)

Gene Editing Services

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Red Cotton Gene knockout Project

OOSP4A Knockout cell line (Hep G2) Order

Catalog#

YKO-HT7596

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

OOSP4A Knockout cell line (Hep G2)

Species

Human

Gene Name

OOSP4A

Gene ID

112577462

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 2 (based on transcript OOSP4A-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the OOSP4A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, OOSP4A gene knockout OOSP4A Knockout cell line (Hep G2) will be expanded and cryopreserved.