PRKDC Knockout cell line (HT-1080)

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PRKDC Knockout cell line (HT-1080) Order

PRKDC Knockout cell line (HT-1080)
Catalog#
YKO-H1433
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PRKDC Knockout cell line (HT-1080)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
PRKDC Knockout cell line (HT-1080)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Gene Name
PRKDC
Gene ID
5591
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 1 (based on transcript PRKDC-201)
Validation: PCR+Sanger Sequencing
PRKDC Knockout cell line (HT-1080) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:HT-1080
STR Info (Cell bank)
Cell Line:HT-1080
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 12141214
D7S820 910910
D16S539 912912
VWA 14191419
TH01 6666
AMEL XYXY
TPOX 8888
CSF1PO 12121212
D12S391 2022
FGA 2225
D2S1338 2526
D21S11 282930.0
D18S51 1218
D8S1179 1314
D3S1358 1616
D6S1043 1218
PENTAE 515
D19S433 13.215
PENTAD 912
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PRKDC gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-1080 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PRKDC gene knockout PRKDC Knockout cell line (HT-1080) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PRKDC Knockout cell line (HT-1080)
PRKDC Knockout cell line (HT-1080)
Catalog#
YKO-H1433
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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