PTPRH Knockout cell line (Hep G2)

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PTPRH Knockout cell line (Hep G2) Order

PTPRH Knockout cell line (Hep G2)
Catalog#
YKO-HT7540
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PTPRH Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PTPRH Knockout cell line (Hep G2)
Species
Human
Gene Name
PTPRH
Gene ID
5794
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 6 (based on transcript PTPRH-202)
Validation: PCR+Sanger Sequencing
PTPRH Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PTPRH gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PTPRH gene knockout PTPRH Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PTPRH Knockout cell line (Hep G2)
PTPRH Knockout cell line (Hep G2)
Catalog#
YKO-HT7540
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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