SELPLG Knockout cell line (Jurkat)

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SELPLG Knockout cell line (Jurkat) Order

SELPLG Knockout cell line (Jurkat)
Catalog#
YKO-H1436
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SELPLG Knockout cell line (Jurkat)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
SELPLG Knockout cell line (Jurkat)
Species
Gene Name
SELPLG
Gene ID
6404
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript SELPLG-203)
Validation: PCR+Sanger Sequencing
SELPLG Knockout cell line (Jurkat) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SELPLG gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SELPLG gene knockout SELPLG Knockout cell line (Jurkat) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SELPLG Knockout cell line (Jurkat)
SELPLG Knockout cell line (Jurkat)
Catalog#
YKO-H1436
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

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