SMIM45 Knockout cell line (Hep G2)

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SMIM45 Knockout cell line (Hep G2) Order

SMIM45 Knockout cell line (Hep G2)
Catalog#
YKO-HT7498
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SMIM45 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SMIM45 Knockout cell line (Hep G2)
Species
Human
Gene Name
SMIM45
Gene ID
339674
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript SMIM45-201)
Validation: PCR+Sanger Sequencing
SMIM45 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SMIM45 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SMIM45 gene knockout SMIM45 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SMIM45 Knockout cell line (Hep G2)
SMIM45 Knockout cell line (Hep G2)
Catalog#
YKO-HT7498
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SMIM45 Knockout cell line (HeLa)

    SMIM45 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93733
    YKO-XN93733(HeLa)
  • SMIM45 Knockout cell line (HCT 116)

    SMIM45 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93732
    YKO-XN93732(HCT 116)
  • SMIM45 Knockout cell line (HEK293)

    SMIM45 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93731
    YKO-XN93731(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SMIM45 Knockout cell line (HeLa)

    SMIM45 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93733
      inquiry   More details
  • SMIM45 Knockout cell line (HCT 116)

    SMIM45 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93732
      inquiry   More details
  • SMIM45 Knockout cell line (HEK293)

    SMIM45 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93731
      inquiry   More details

SMIM45 Knockout cell line (Hep G2) Order

SMIM45 Knockout cell line (Hep G2)
Catalog#
YKO-HT7498
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SMIM45 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SMIM45 Knockout cell line (Hep G2)
Species
Human
Gene Name
SMIM45
Gene ID
339674
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript SMIM45-201)
Validation: PCR+Sanger Sequencing
SMIM45 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SMIM45 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SMIM45 gene knockout SMIM45 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SMIM45 Knockout cell line (Hep G2)
SMIM45 Knockout cell line (Hep G2)
Catalog#
YKO-HT7498
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SMIM45 Knockout cell line (HeLa)

    SMIM45 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93733
    YKO-XN93733(HeLa)
  • SMIM45 Knockout cell line (HCT 116)

    SMIM45 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93732
    YKO-XN93732(HCT 116)
  • SMIM45 Knockout cell line (HEK293)

    SMIM45 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93731
    YKO-XN93731(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SMIM45 Knockout cell line (HeLa)

    SMIM45 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93733
      inquiry   More details
  • SMIM45 Knockout cell line (HCT 116)

    SMIM45 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93732
      inquiry   More details
  • SMIM45 Knockout cell line (HEK293)

    SMIM45 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: SMIM45
    Gene ID: 339674
    Size: 1*10^6
    Catalog#: YKO-XN93731
      inquiry   More details

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