THSD8 Knockout cell line (Hep G2)

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location： Home > Products > KO Cell Lines > THSD8 Knockout cell line (Hep G2)

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THSD8 Knockout cell line (Hep G2) Order

THSD8 Knockout cell line (Hep G2)
Catalog#
YKO-HT7408
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
THSD8 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
THSD8 Knockout cell line (Hep G2)
Species
Human
Gene Name
THSD8
Gene ID
111644133
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript THSD8-202)
Validation: PCR+Sanger Sequencing
THSD8 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the THSD8 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, THSD8 gene knockout THSD8 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
THSD8 Knockout cell line (Hep G2)
THSD8 Knockout cell line (Hep G2)
Catalog#
YKO-HT7408
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • THSD8 Knockout cell line (HeLa)

    THSD8 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94580
    YKO-XN94580(HeLa)
  • THSD8 Knockout cell line (HCT 116)

    THSD8 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94579
    YKO-XN94579(HCT 116)
  • THSD8 Knockout cell line (A549)

    THSD8 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94577
    YKO-XN94577(A549)

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Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • THSD8 Knockout cell line (HeLa)

    THSD8 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94580
      inquiry   More details
  • THSD8 Knockout cell line (HCT 116)

    THSD8 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94579
      inquiry   More details
  • THSD8 Knockout cell line (A549)

    THSD8 Knockout cell line (A549)

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94577
      inquiry   More details

THSD8 Knockout cell line (Hep G2) Order

THSD8 Knockout cell line (Hep G2)
Catalog#
YKO-HT7408
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
THSD8 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
THSD8 Knockout cell line (Hep G2)
Species
Human
Gene Name
THSD8
Gene ID
111644133
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript THSD8-202)
Validation: PCR+Sanger Sequencing
THSD8 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the THSD8 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, THSD8 gene knockout THSD8 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
THSD8 Knockout cell line (Hep G2)
THSD8 Knockout cell line (Hep G2)
Catalog#
YKO-HT7408
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • THSD8 Knockout cell line (HeLa)

    THSD8 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94580
    YKO-XN94580(HeLa)
  • THSD8 Knockout cell line (HCT 116)

    THSD8 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94579
    YKO-XN94579(HCT 116)
  • THSD8 Knockout cell line (A549)

    THSD8 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94577
    YKO-XN94577(A549)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • THSD8 Knockout cell line (HeLa)

    THSD8 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94580
      inquiry   More details
  • THSD8 Knockout cell line (HCT 116)

    THSD8 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94579
      inquiry   More details
  • THSD8 Knockout cell line (A549)

    THSD8 Knockout cell line (A549)

    Organism: Human
    Gene Name: THSD8
    Gene ID: 111644133
    Size: 1*10^6
    Catalog#: YKO-XN94577
      inquiry   More details

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