VMA21 Knockout cell line (Hep G2)

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VMA21 Knockout cell line (Hep G2) Order

VMA21 Knockout cell line (Hep G2)
Catalog#
YKO-HT7630
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
VMA21 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
VMA21 Knockout cell line (Hep G2)
Species
Human
Gene Name
VMA21
Gene ID
203547
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript VMA21-201)
Validation: PCR+Sanger Sequencing
VMA21 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the VMA21 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, VMA21 gene knockout VMA21 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
VMA21 Knockout cell line (Hep G2)
VMA21 Knockout cell line (Hep G2)
Catalog#
YKO-HT7630
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

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  • VMA21 Knockout cell line (HeLa)

    VMA21 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33856
    YKO-XN33856(HeLa)
  • VMA21 Knockout cell line (HCT 116)

    VMA21 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33855
    YKO-XN33855(HCT 116)
  • VMA21 Knockout cell line (HEK293)

    VMA21 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33854
    YKO-XN33854(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • VMA21 Knockout cell line (HeLa)

    VMA21 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33856
      inquiry   More details
  • VMA21 Knockout cell line (HCT 116)

    VMA21 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33855
      inquiry   More details
  • VMA21 Knockout cell line (HEK293)

    VMA21 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33854
      inquiry   More details

VMA21 Knockout cell line (Hep G2) Order

VMA21 Knockout cell line (Hep G2)
Catalog#
YKO-HT7630
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
VMA21 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
VMA21 Knockout cell line (Hep G2)
Species
Human
Gene Name
VMA21
Gene ID
203547
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript VMA21-201)
Validation: PCR+Sanger Sequencing
VMA21 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the VMA21 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, VMA21 gene knockout VMA21 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
VMA21 Knockout cell line (Hep G2)
VMA21 Knockout cell line (Hep G2)
Catalog#
YKO-HT7630
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • VMA21 Knockout cell line (HeLa)

    VMA21 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33856
    YKO-XN33856(HeLa)
  • VMA21 Knockout cell line (HCT 116)

    VMA21 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33855
    YKO-XN33855(HCT 116)
  • VMA21 Knockout cell line (HEK293)

    VMA21 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33854
    YKO-XN33854(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • VMA21 Knockout cell line (HeLa)

    VMA21 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33856
      inquiry   More details
  • VMA21 Knockout cell line (HCT 116)

    VMA21 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33855
      inquiry   More details
  • VMA21 Knockout cell line (HEK293)

    VMA21 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: VMA21
    Gene ID: 203547
    Size: 1*10^6
    Catalog#: YKO-XN33854
      inquiry   More details

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