POLE3 Knockout cell line (NCI-H82)

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POLE3 Knockout cell line (NCI-H82) Order

POLE3 Knockout cell line (NCI-H82)
Catalog#
YKO-HA100500
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
POLE3 Knockout cell line (NCI-H82)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
POLE3 Knockout cell line (NCI-H82)
Species
Human
Gene Name
POLE3
Gene ID
54107
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the POLE3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NCI-H82 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, POLE3 gene knockout POLE3 Knockout cell line (NCI-H82) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
POLE3 Knockout cell line (NCI-H82)
POLE3 Knockout cell line (NCI-H82)
Catalog#
YKO-HA100500
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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