PSPC1 Knockout cell line (Jurkat)

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PSPC1 Knockout cell line (Jurkat) Order

PSPC1 Knockout cell line (Jurkat)
Catalog#
YKO-HT12501
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PSPC1 Knockout cell line (Jurkat)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
PSPC1 Knockout cell line (Jurkat)
Species
Human
Gene Name
PSPC1
Gene ID
55269
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PSPC1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PSPC1 gene knockout PSPC1 Knockout cell line (Jurkat) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PSPC1 Knockout cell line (Jurkat)
PSPC1 Knockout cell line (Jurkat)
Catalog#
YKO-HT12501
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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