TMEM159 Knockout cell line (Caco-2)

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TMEM159 Knockout cell line (Caco-2) Order

TMEM159 Knockout cell line (Caco-2)
Catalog#
YKO-HA170612
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TMEM159 Knockout cell line (Caco-2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
TMEM159 Knockout cell line (Caco-2)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
TMEM159
Gene ID
57146
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
77%MEM+20%FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Caco-2		 STR Info (Cell bank)
Cell Line:CACO-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 1114111413.0
D7S820 11121112
D16S539 12121213
VWA 16181618
TH01 6666
AMEL XXXX
TPOX 911911
CSF1PO 11111111
D12S391 1723
FGA 1919
D2S1338 1725
D21S11 3030
D18S51 1212
D8S1179 1212
D3S1358 1717
D6S1043 1212
PENTAE 77
D19S433 1515
PENTAD 99
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM159 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Caco-2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM159 gene knockout TMEM159 Knockout cell line (Caco-2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TMEM159 Knockout cell line (Caco-2)
TMEM159 Knockout cell line (Caco-2)
Catalog#
YKO-HA170612
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Gene ID: 57146
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    Request a Quote More details
  • TMEM159 Knockout cell line (RT4)

    TMEM159 Knockout cell line (RT4)

    Organism: Human
    Gene Name: TMEM159
    Gene ID: 57146
    Size: 1*10^6
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    Request a Quote More details
  • TMEM159 Knockout cell line (ACHN)

    TMEM159 Knockout cell line (ACHN)

    Organism: Human
    Gene Name: TMEM159
    Gene ID: 57146
    Size: 1*10^6
    Catalog#: YKO-HA159446
    Request a Quote More details

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