TMEM159 Knockout cell line (RT4)

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TMEM159 Knockout cell line (RT4) Order

TMEM159 Knockout cell line (RT4)
Catalog#
YKO-HA165150
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TMEM159 Knockout cell line (RT4)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
TMEM159 Knockout cell line (RT4)
Cell Morphology
Epithelial-like, adheren
Passage ratio
1:2~1:3
Species
Human
Gene Name
TMEM159
Gene ID
57146
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% McCOY's 5A +10%FBS
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:RT4		 STR Info (Cell bank)
Cell Line:RT-4
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 8888
D7S820 912912
D16S539 9999
VWA 14171417
TH01 99.399.3
AMEL XYXY
TPOX 811811
CSF1PO 10121012
D12S391 2022
FGA 2224
D2S1338 1819
D21S11 3032.2
D18S51 1517
D8S1179 1315
D3S1358 1515
D6S1043 1319
PENTAE 710
D19S433 1313
PENTAD 1213
D1S1656 1415
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM159 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RT4 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM159 gene knockout TMEM159 Knockout cell line (RT4) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TMEM159 Knockout cell line (RT4)
TMEM159 Knockout cell line (RT4)
Catalog#
YKO-HA165150
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Organism: Human
    Gene Name: TMEM159
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    Gene ID: 57146
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Related Popular Products

Wild-type Cell Lines

KO Cell Lines

  • TMEM159 Knockout cell line (PC-9)

    TMEM159 Knockout cell line (PC-9)

    Organism: Human
    Gene Name: TMEM159
    Gene ID: 57146
    Size: 1*10^6
    Catalog#: YKO-HA175892
    Request a Quote More details
  • TMEM159 Knockout cell line (Caco-2)

    TMEM159 Knockout cell line (Caco-2)

    Organism: Human
    Gene Name: TMEM159
    Gene ID: 57146
    Size: 1*10^6
    Catalog#: YKO-HA170612
    Request a Quote More details
  • TMEM159 Knockout cell line (ACHN)

    TMEM159 Knockout cell line (ACHN)

    Organism: Human
    Gene Name: TMEM159
    Gene ID: 57146
    Size: 1*10^6
    Catalog#: YKO-HA159446
    Request a Quote More details

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