Name: Monoclone Genotype Validation Kit (extraction free)
SKU: ~100 tests
Price: 300 USD
Availability: InStock

EZ-editor™ Gene-editing Series Products

With 15 years of experience, Ubigene has exclusively innovated and developed 6 product lines, fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved, achieving our aim of Make genome editing easier!

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Monoclone Genotype Validation Kit (extraction free)

This kit is developed for monoclone genotype validation in the process of generating gene-editing cell lines. The validation kit can be used in early growth stage of clones and can screen qualified single clones in advance, and eliminate the cost of culturing large numbers of non-positive clones. It can also shorten the turnaround of 2-4 weeks. The preparation of the genome samples of single-cell clones only takes 15-20 mins. The cells are directly lysed to release the genome and the cell lysate can be used directly in following PCR experiments without purification.Utilizing 96-well plates to simultaneously identify up to hundreds of clones makes high-throughput validation of single-cell clones.

Classic version

Preferred PCR validation reagent, CloneAmp Taq Mix (+ Dye), are compatible with residual media from crude extracted genomic samples and components after cell lysis that may affect PCR reactions, ensuring validation results can be obtained accurately and quickly.

After a large number of internal tests, the kit can be used for adherent cells, suspension cells, including but not limited to cells of human, mouse, rat or other animal origin. The recommended sample size is 3x103 ~ 105 cells (generally 1x104/well for 96-well plate), and the minimum sample size is not less than 100 cells.

Additionally, Ubigene has released two useful tools (1) the primer design tool for gene editing target site identification (EZ-editor™ high efficiency primer design platform) provided in the Red Cotton™ system and (2) EZ-editor™ Genotype Analysis System (GAS)can assist you more rapidly through the entire single-cell clone validation process.

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Kit Components

Product Advantage

Easy to use

Only 3 steps for the preparation of genomic samples; The cell lysate can be used directly in following PCR experiments without purification; Utilizing 96-well plates to simultaneously identify up to hundreds of clones makes high-throughput validation of single-cell clones.

Micro usage of cells

As low as 30-50 cells required, and their genotypes can be identified at the early growth stage of clones, which greatly reduces the experimental turnaround and reduces the cost of experiments.

Long term storage

The amount of DNA template contained in the lysate can be used in 20 ~ 30 PCR experiments and can be kept in a low temperature environment for several months, which can meet the need of multiple repeated validation.

Preferred reagents

Monoclone Genotype Validation Kit (extraction free) contains preferred PCR validation reagents that are compatible with residual media from crude extracted genomic samples and components after cell lysis, and so on that may affect PCR reactions, ensuring validation results can be obtained accurately and quickly.

Broad applications

Applicable to all types of adherent versus suspension cells,including but not limited to cells of human, mouse, rat or other animal origin, and supports all types of gene editing experiments for positive clone validation.