PBK Knockout cell line(U-2OS)

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Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

PBK Knockout cell line(U-2OS) Order

Catalog#：YKO-H537

Price (USD)：-

Size： 1*10^6

Instruction：

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YKO-H537
Cell line	PBK Knockout cell line(U-2 OS)	Cell Type	Mouse Lewis lung carcinoma Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:3~1:5
Culture method	90% McCOY's 5A+10%FBS
Cryopreservation solution	50%McCOY's 5A+40%FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol

PBK

Gene id

55872

Official full symbol

PDZ binding kinase

Also known as

CT84, HEL164, Nori-3, SPK, TOPK

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants.