Gene Editing Services / Stable Cell Lines

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  • Knockout Cell Lines

    1. Technical Details CRISPR/Cas9 system is a versatile tool for gene modification of nearly all types of cells. crRNA (CRISPR-derived RNA) binds with tracr-RNA (trans-activating RNA) by base pairing to form tracrRNA/crRNA complex, which guides the Cas nuclease to cleave and introd…

    2020-03-16
  • Knockdown Cell Lines

    The knockdown vector would be transferred into the cells by lentivirus transduction. After drug screening, WT cells die out and a positive cell pool obtained.

    2020-03-16
  • Overexpression Cell Lines

    The overexpression vector would be transferred into the cells by lentivirus transduction or piggyBac transposition. After drug screening, WT cells die out and a positive cell pool obtained.

    2020-03-16
  • Knockin Cell Lines

    The cells would be co-transfected with gRNA, Cas9 and donor vector by electroporation, and then screened. After drug screening, single clones would be generated. Positive clones would be validated by sequencing. Cells knockin with reporter genes can also be selected by observing t…

    2020-03-16
  • Point Mutation Cell Line

    The cells would be co-transfected with gRNA, Cas9 and donor oligo by electroporation, and then screened. After drug screening, single clones would be generated. Positive clones would be validated by sequencing.

    2020-03-16
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