Red/ET recombination system is a classical method of microbial gene editing, which can achieve the knockin, knockout, point mutation and other modifications of the target gene. This technology has been widely used in genetic modification of genomic DNA, such as bacterial artificial chromosome(BAC), Escherichia coli chromosome. However, the efficiency of this system still needs to be improved.
How to improve the efficiency of gene recombination and editing has always been a hotspot of microbial gene editing. Therefore, CRISPR/Cas9 technology is adopted to improve the efficiency of microbial gene editing.
The efficiency is 20-30 times higher than that of the classic methods;
Scarless gene-editing technology, safe and sound;
Easily achieve microbial gene knockout (KO), point mutation (PM) and knockin (KI);
It is possible to knockout multiple genes simultaneously.
Ubigene uses CRISPR-B™ system to modify the genome of fungi and achieve knockout, point mutation or knockin. Customers could choose the genome editing methods with or without residual.
What fungus can CRISPR-B™ modify?
· Pichia pastoris
· Saccharomyces cerevisiae
· Aspergillus niger
· Trichoderma reesei
· Aspergillus fumigatus
· Aspergillus nidulans
· some Fusarium
For more fungi species, please consult us.
1. Colony PCR 2.Sequencing