This EZ-editor™ Gene Knockout kit is based on the CRISPR-U™ system, and provides the main ingredients required in the whole process of gene-editing. The EZ-editor™Gene Knockout kit provides efficient and convenient gene knockout tools for researchers and achieves the fast construction of gene knockout cell lines.
In order to achieve efficient gene knockout, 3 knockout plasmids of EZ-editor™ gRNAs in this kit are constructed by Ubigene Red Cotton™ CRISPR Gene Editing System after high-throughput data simulation. These knockout plasmids can ensure the comprehensively optimal performance on the cutting site, cutting efficiency, and specificity.
To greatly reduce the experimental cost and shorten the experimental turnarounds. The MicroCell DNA Lysis and PCR Validation in the EZ-editor™ Gene Knockout kit are the developed products for single-cell clones validation in gene-editing experiment, which can validate the single-cell clones at the early stage of single-cell clone growth. The preparation of genomic samples only takes 15-20 minutes. The cells are directly lysed to release the genome and the cell lysate can be used directly in following PCR experiments without purification. Utilizing 96-well plates to simultaneously identify up to hundreds of clones makes high-throughput validation of single-cell clones. Through screening positive single-cell clones at early stage, the experimental turnarounds is shortened by 2-4 weeks, and the experimental cost of culturing non-positive clones are greatly reduced. The preferred PCR validation reagent can be compatible with the factors that may affect the PCR reaction, such as the residual medium and components after cell lysis in the roughly extracted genome samples, and can quickly and accurately identify the genotype of single-cell clones.
Additionally, Ubigene has released two useful tools (1) the primer design tool for gene editing target site identification (EZ-editor™ high efficiency primer design platform) provided in the Red Cotton™ system and (2) EZ-editor™ Genotype Analysis System (GAS) can assist you more rapidly through the entire single-cell clone validation process.