CRISPR Library Virus & Cell Pool
CRISPR library cell pools are generated by infecting target cells with a CRISPR library viral containing diverse sgRNAs at a low infect MOI. This approach creates a cell pool, each carrying different sgRNAs and exhibiting distinct gene knockout backgrounds.By applying specific selection pressures—such as drug treatment, serial passaging, or flow cytometry sorting—cell subpopulations with particular phenotypes can be enriched or depleted. Subsequent next-generation sequencing (NGS) enables the systematic identification of key genes associated with these phenotypes.This technology is widely used for: Investigating mechanisms of drug resistance,studying tumor metastasis and invasion,Elucidating immune interactions and regulatory mechanisms,or genome-wide or customized target screening.
Why choose Ubigene?
Why choose Ubigene?
Extensive In-Stock Resources
High-Titer and Uniform Virus
High Cell Coverage, Low infect MOI
Robust Quality Assurance
Flexible Service Options
Workflow
gRNA Library Design & Plasmid Construction
Lentiviral Packaging & Titer Assessment
MOI Optimization & Cell Pool Infection
Antibiotic Selection & Cell Pool Generation
NGS Sequencing & Data Analysis
CRISPR Library Virus & Cell Pool Services Overview
CRISPR Library Virus Service
In-Stock CRISPR Library Virus
After plasmid library amplification, lentiviral packaging, virus concentration, and titer assessment are performed to ensure a viral titer > 1 * 10⁸ TU/mL.
Turnaround
1 week
Deliverables
Corresponding CRISPR library virus and COA report.
Custom CRISPR Library Virus
sgRNAs are designed de novo, and a custom plasmid library is constructed. Following plasmid amplification and NGS validation, lentiviral packaging, concentration, and titer assessment are performed to ensure a viral titer > 1 * 10⁸ TU/mL.
Turnaround
6-9 weeks
Deliverables
Corresponding CRISPR library virus, COA report, and NGS sequencing report of the custom CRISPR library plasmid.
CRISPR Library Cell Pool Service
In-Stock Screening-ready Cell Pool
Screening-ready Cell Pools. Cells undergo thaw recovery, bacterial and mycoplasma testing, and NGS validation to ensure quality before delivery.
Turnaround
1 week
Deliverables
CRISPR Library Cell Pool with ≥300* or 500* coverage, NGS sequencing report
CRISPR Library Cell Pool in Development
Using in-stock CRISPR library viruses, target cells are subjected to MOI optimization, viral infection, drug selection, and NGS sequencing to generate CRISPR library cell pools.
Turnaround
6-9 weeks
Deliverables
CRISPR Library Cell Pool with ≥300* or 500* coverage, NGS sequencing report
Custom CRISPR Library Cell Pool
Design sgRNAs, construct a custom plasmid library, and package it into lentivirus. Next, perform MOI optimization, viral infection, drug selection, and NGS-based quality control to generate a fully
Turnaround
11-13 weeks
Deliverables
CRISPR Library Cell Pool with ≥300* or 500* coverage, NGS sequencing report
Case Studies
1. CRISPR Library Virus Packaging & Titer Assessment
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2. MOI Optimization for cell Infection
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3. Cell Images
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4. NGS Sequencing of CRISPR Library Cell Pool for sgRNA Coverage
1.Next-generation sequencing (NGS) results for custom CRISPR library cell pool.
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2.Next-generation sequencing (NGS) results of whole-genome knockout library cell pool.
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FAQs
1. Why is the MOI for 30% virus infection efficiency chosen as the viral infection condition for library cell construction experiments?
- An MOI of 0.3 is optimal for CRISPR screening:
- Single-Gene Editing: Ensures each cell is infected by only one viral particle, minimizing multiple sgRNA expression.
- Efficiency and Viability: Achieves 30% infection without significant toxicity, maintaining cell health.
- Coverage: Ensures sufficient sgRNA expression for robust screening results.
- Practicality: Reduces viral particle use, lowers cost, and simplifies procedures.
