CRISPR-iScreen™ NGS Sequencing

CRISPR library screening enables large-scale functional genomics studies, allowing researchers to systematically investigate gene functions at the genome-wide level. Following the completion of a screening experiment, next-generation sequencing (NGS) is typically employed to measure the abundance of each sgRNA in experimental and control groups.By comparing sgRNA read counts across different conditions and applying mainstream bioinformatics algorithms such as MAGeCK or DrugZ, researchers can perform quantitative analysis of sgRNA enrichment or depletion, efficiently identifying candidate genes closely associated with specific biological processes or phenotypes.This strategy has been widely applied in cutting-edge research areas, including gene function annotation, drug target discovery, mechanisms of drug resistance, and cancer development and progression studies.

Why choose Ubigene?

Comprehensive End-to-End Services

Quality control standards are implemented at every stage of the workflow—from sample receipt to library construction, sequencing, and data analysis—to ensure the accuracy, reliability, and reproducibility of the results.

High Sequencing Depth and Data Volume

We provide a sequencing depth of ≥300*, ensuring comprehensive coverage of sgRNAs and minimizing potential biases caused by insufficient sequencing.

iScreenAnlys™CRISPR Library Analysis Platform

Multiple statistical methods and visualization outputs
Customizable analysis pipelines tailored to experimental needs
One-click generation of publication-ready figures and reports

Professional Scientific Support

Our team of experienced bioinformaticians and molecular biologists provides comprehensive technical support and result interpretation, assisting researchers in rapidly identifying key targets and insights from their studies.

NGS Sequencing & Workflow

Genomic DNA Extraction from cells

PCR Amplification of target regions

Gel Purification of PCR products

NGS Library Preparation

NGS Sequencing

Sequencing Data Processing

Bioinformatics Analysis

Data Upload to iScreenAnlys™ CRISPR Library Analysis Platform

iScreenAnlys™ CRISPR Library Analysis Platform

Ubigene developed the iScreenAnlys™ CRISPR Library Analysis Platform, enabling “one-click analysis” of CRISPR library screening data. The platform fully integrates MAGeCK statistical workflows and quality control modules, automatically performing deep data parsing, QC, and result annotation, and generating publication-ready visualizations that meet international journal standards.

Moreover, the platform supports multi-dimensional, customizable analyses and visualizations. Even users without programming experience can easily operate the system, efficiently unlocking the value of their data, identifying candidate genes, and redefining the efficiency of CRISPR library screening analysis.

*For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.