Comprehensive CRISPR Gene Editing Solutions to Power Your Research: KO Cell Lines, CRISPR Screen & More

Incubate sgRNA and Cas9 protein in vitro to form an RNP (ribonucleoprotein) complex, which is then co-transfected into cells along with a single-stranded oligonucleotide. After the RNP induces a double-strand break at the target site, the oligonucleotide can introduce the desired mutation into the genomic target through homologous recombination.

Features:

• Broad applicability
• High editing efficiency
• Short turnaround time

Applicable Types:

• Suitable when a gRNA sequence can be identified within approximately 10 bp of the mutation site.
• Applicable to target cells that can be transfected using electroporation or liposome-based transfection.

Gene editing is achieved by fusing an engineered reverse transcriptase with a Cas9 nickase and using a special prime editing guide RNA (pegRNA).

Features:

• Capable of correcting various complex mutations.
• However, it has low editing efficiency and requires complex vector design.

Applicable Types:

• Suitable for correcting complex mutations.
• Ideal for cases where an appropriate gRNA sequence cannot be found near the mutation site.

A base editing system is developed by integrating a base deaminase with the CRISPR/Cas system, enabling precise introduction of C/G-to-T/A and A/T-to-G/C point mutations without creating double-strand breaks, thereby achieving highly efficient and accurate gene editing.

Features:

• High efficiency, but applicable cases are limited.

Applicable Types:

• Suitable for specific single-base conversions (C⇌T; A⇌G) when the target site is within the active window.

The donor vector is constructed using a plasmid, with a resistance gene expression cassette inserted into an intron to enhance the efficiency of point mutation recombination through antibiotic screening.

Features:

• Suitable for scenarios where a suitable gRNA sequence cannot be identified near the mutation site; however, it is relatively expensive and has a longer experimental cycle.

Applicable Types:

• Ideal for cases where a suitable gRNA sequence cannot be identified near the mutation site.