CRISPR libraries are collections of sgRNAs designed to target multiple genes using the CRISPR/Cas9 system. By targeting thousands of genes in parallel, CRISPR library screening helps researchers study gene function and identify potential targets involved in specific biological processes. After screening and selection, NGS and bioinformatic data analysis can be used to measure sgRNA enrichment and depletion and identify genes for further validation.
Exploring the CRISPR/Cas9 Library: Precision Targeting to Uncover Essential Genes>>
Powered by our proprietary EZ-iScreen™ technology, Ubigene provides end-to-end CRISPR library and screening solutions, from custom sgRNA library design and construction to lentiviral packaging, cell transduction, in vitro or in vivo screening, NGS, and bioinformatics analysis. Library types include CRISPR-KO, CRISPRa, and CRISPRi libraries to meet different needs. For researchers who prefer to run their own screening workflows, we also offer pre-made CRISPR libraries, including plasmid and pre-packaged lentivirus, transduced cell pool (ready-to-screen) formats. Multiple library formats and delivery options are available depending on your research needs.
CRISPR Library & Screening Services Overview
CRISPR Library Construction Services
Ubigene offers end-to-end custom library construction—from gRNA design and oligo synthesis to vector assembly, lentiviral packaging, transduction, and NGS validation—with >99% coverage. Off-the-shelf products include 40+ plasmid libraries, 150+ viral libraries, and 1,000+ pooled cell libraries.
CRISPR Library Construction Services
Ubigene offers end-to-end custom library construction—from gRNA design and oligo synthesis to vector assembly, lentiviral packaging, transduction, and NGS validation—with >99% coverage. Off-the-shelf products include 40+ plasmid libraries, 150+ viral libraries, and 1,000+ pooled cell libraries.
CRISPR Library Construction Services
Ubigene offers end-to-end custom library construction—from gRNA design and oligo synthesis to vector assembly, lentiviral packaging, transduction, and NGS validation—with >99% coverage. Off-the-shelf products include 40+ plasmid libraries, 150+ viral libraries, and 1,000+ pooled cell libraries.
Ubigene EZ-iScreen™ Technique
A technology self-developed by Ubigene, designed to enable efficient and precise CRISPR screening, providing researchers a powerful and reliable tool for gene function studies and drug target discovery.
High-Efficiency Competent Cells
Specially developed for CRISPR library construction, optimizing for exogenous DNA uptake and achieving efficient transformation with low mutagenesis risk. This ensures reliable plasmid library amplification, consistently delivering >99% coverage and <10 uniformity.
Diverse CRISPR Screening Platforms
Flexible in vitro and in vivo screening platforms support diverse experimental conditions and screening strategies, allowing researchers to customize screening workflows to their specific needs.
Optimization of transduction protocols for target cell libraries
Enabling scalable, standardized production of pooled target cell library with batch-to-batch consistency, high reproducibility, and up to 99% library coverage.
iScreenAnlys™ CRISPR Library Analysis Platform
An intuitive, user-friendly platform for CRISPR screening data analysis, with customizable statistical methods and visualizations for fast, publication-ready results.
CRISPR Screening Workflow
gRNA design
Chip synthesis of Oligo Pool
Plasmid construction
Lentivirus packaging
Cell Pool Construction (Cell infection by lentiviral library)
(Positive or negative screening by applying screening pressure)
PCR amplification
Sequencing
Analyze the data and find out candidates
FAQs
1. Why is CRISPR screening important for gene function studies?
Traditional gene function studies mainly rely on forward genetics and reverse genetics. Forward genetics, often based on high-throughput multi-omics analyses, is expensive and makes it difficult to establish a direct link between gene targets and phenotypes. Reverse genetics involves direct gene perturbation at the cellular or organismal level, enabling clear causal relationships between genes and phenotypes. However, it can be costly, labor-intensive, and heavily dependent on prior knowledge. CRISPR library screening combines the high-throughput capacity of forward genetics with the causal clarity of reverse genetics, allowing researchers to unbiasedly identify phenotype-associated functional targets from large gene sets. This makes it a powerful and efficient tool for large-scale functional genomics research.
2. What are the key advantages of CRISPR library screening?
- Establishes a direct causal relationship between genes and phenotypes
- Enables unbiased, systematic functional exploration across a large number of genes
- Flexible and highly customizable screening systems
- Scalable with broad applicability of screening data
- Cost-effective and efficient for large-scale functional studies
- Features high specificity and low off-target effects
3. What research areas commonly use CRISPR library screening?
CRISPR screening is widely applied in oncogenesis, drug resistance, and viral infection studies to identify key genetic targets. It is also used to explore unknown gene functions in areas such as signaling pathways, immunology, and metabolism. In addition, CRISPR library screening can be adapted and optimized for more complex research systems, including stem cell models and aging studies, expanding its potential across a broad range of biological and disease research fields.
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