Gene Overexpression Cell Line Service
Gene overexpression cell lines are important tools for studying gene function, disease mechanisms, and drug action. By increasing the expression level of a specific gene, they enable functional enhancement of the gene. They can also be applied in rescue experiments to validate phenotypic recovery following gene knockout.
Overexpression vectors are introduced into cells via lentiviral infection or nucleofection. Cells are then subjected to compound selection based on an optimized concentration until all control cells are eliminated, resulting in stable gene-expressing cell lines. Ubigenetech's proprietary EZ-OE™ technology differs from conventional lentiviral or transposon-based methods by enabling site-specific genomic integration and single-copy insertion, while offering lower cost and faster turnaround.
Application
Learn More: Application of Overexpression Cell Lines in Colorectal Cancer Research
EZ-OE™ Technology introduction
Ubigene's proprietary EZ-OE™ technology is a genome engineering platform that enables precise and stable integration of exogenous DNA without the use of viruses, ensuring safe and efficient gene expression. This technology provides research and industrial customers with safe, stable, and high-expressing cell lines, accelerating gene function studies, protein expression optimization, and the screening and evaluation of candidate therapeutics.
Multiple Vector Systems Available
A variety of transfection methods are available, including lentiviral, plasmid, and electroporation, providing flexible options to match different cell types.
Selectable Markers: EGFP, mCherry, Luciferase, Puromycin, Neomycin, and others.
Extensive Experience
Over 300 successful gene editing projects delivered, providing stable and reliable overexpression cell lines.
Comprehensive Quality Control
Multi-dimensional validation, including RT-qPCR, ensures accurate gene overexpression or knockdown efficiency.
Broad Cell Type Coverage
Compatible with cancer cell lines and standard cell types, supporting a wide range of experimental models.
Overexpression Cell Line Service
Cell type
Various types of cells including tumor cell lines, regular cell lines
Deliverables
Cell pool/Single-cell clone
Turnaround
Please Inquire
Price
Start from $2145
*For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Contact Us 300+ Successful Gene-editing Cell Line Types
Digestive System
Human Gallbladder Carcinoma Cell Line (GBC-SD)Human Hepatoma Cell Line (Hep G2)Human Hepatoma Cell Line (SNU-387)Human Hepatoma Cell Line (HuH-7)Human Hepatoma Cell Line (SK-HEP-1)Human Hepatoma Cell Line (Hep 3B)Human Normal Hepatocytes Cell line (L-02)Human Colon Cancer Cell Line (HCT 116)Human Colon Cancer Cell Line (HT-29)Human colorectal adenocarcinoma Cell Line (RKO)Human colorectal adenocarcinoma Cell Line (Caco-2)Human Esophageal Squamous Carcinoma Cell Line (KYSE-150)Human gastric cancer Cell Line (AGS)Mouse Hepatocarcinoma Cell Line (Hepa 1-6)Murine colorectal carcinoma Cell Line (CT26.WT)Murine colorectal carcinoma Cell Line (MC38)Human Esophageal Squamous Carcinoma Cell Line (KYSE-30)Mouse Hepatocarcinoma Cell Line (H22)Human Gastric Cancer Cell Line (NCI-N87)Human colorectal adenocarcinoma Cell Line (SW480)Human Hepatoma Cell Line (SMMC-7721)Porcine intestinal epithelial Cell Line (IPEC-J2)Human Gastric Cancer Cell Line (HGC-27)Human Gastric Cancer Cell Line (MGC-803)Human renal carcinoma cell line (ACHN)Human colorectal adenocarcinoma Cell Line (LS 174T)Immortalized Human Hepatic Cell Line (C3A)Human Hepatoma Cell Line (PLC/PRF/5)Human colorectal adenocarcinoma Cell Line (SW 48)Human colorectal adenocarcinoma Cell Line (SW948)Human colorectal adenocarcinoma Cell Line (NCI-H716)Mouse hepatocyte Cell Line (AML12)Human Gastric Cancer Cell Line (SGC-7901)Human Ileocecal Cancer Cell Line (HRT-18)Human Colon Cancer Cell Line (LoVo)Human Hepatoma Cell Line (MHCC97-H)Human Gastric Cancer Cell Line (MKN-45)Human Colon Cancer Cell Line (COLO 205)Rat Liver Cell Line (BRL)Mouse Gastric Cancer Cell Line (MFC)Human Colon Cancer Cell Line (SW620)Human Hepatoma Cell Line (Li-7)Mouse Ascites Sarcoma Cell Line (S180)Rat Liver Cell Line (BRL-3A)Human Colon Cancer Cell Line (HCT-15)Human Gastric Cancer Cell Line (KATO III)Human colorectal adenocarcinoma Cell Line (SW1463)Human Gastric Cancer Cell Line (SNU-5)Human colorectal adenocarcinoma Cell Line (SW1116 [SW 1116, SW-1116])Human Hepatic Stellate Cell Line (LX-2)Human Hepatoma Cell Line (HCCLM3)
Endocrine System
Human Submandibular Gland Epidermoid Carcinoma Cell Line (A-253)Human Mammary Epithelial Cell Line (MCF 10A)Human Breast Cancer Cell Line (MDA-MB-231)Human Breast Cancer Cell Line (MCF7)Human Breast Cancer Cell Line (MDA-MB-415)Human Breast Cancer Cell Line (MDA-MB-468)Human Breast Epithelial Cell Line Integrating SV40 Gene (HBL-100)Human Prostate Cancer Cell Line (LNCaP Clone FGC)Human Prostate Cancer Cell Line (PC-3)Human Prostate Cancer Cell Line (22RV1)Human pancreatic cancer Cell Line (BxPC-3)Mouse Breast Cancer Cell Line (4T1)Mouse Acinar Pancreatic Cell Line (266-6)Human Breast Cancer Cell Line (JIMT-1)Mouse prostate cancer Cell Line (RM-1)Human Breast Cancer Cell Line (SK-BR-3)Human Breast Cancer Cell Line (HCC1806)Human Thyroid Normal Cell Line (Nthy-ori 3-1)Mouse pancreatic cancer Cell Line (Pan-02)Human Pancreatic Carcinoma Cell Line (SW1990)Human acute lymphoblastic leukemia Cell Line (MOLT4)Human Pancreatic Carcinoma Cell Line (Panc-1)Mouse Breast Adenocarcinoma Cell Line (E0771)Human Thyroid Squamous Cell Carcinoma Cell line (SW579)Human Breast Cancer Cell Line (HCC1143)Human breast cancer Cell Line (BT-549)Human acute lymphoblastic leukemia Cell Line (MT-4)Py230 Cell Line (Py230)Human Thyroid Cancer Cell Line (TPC-1)Human Prostate Cancer Cell Line (PC-3M)Human Burkitt's Lymphoma Cell Line(RAJI)Human Metastatic Pancreatic Adenocarcinoma Cell Line (AsPC-1)Human NK Leukemia Cell Line (YT)Human Prostate Cancer Cell Line (DU145)Mouse Mammary Epithelial Cell Line (HC11)Mouse Breast Cancer Cell Line (C127)Human Thyroid Normal Cell Line (Nthy-ori 3-1)Human Pancreatic Carcinoma Cell Line (MIA PaCa-2)Mouse Breast Cancer Cell Line (EMT6)Human Prostate Cancer Cell Line (VCaP)Human Breast Cancer Cell Line (SUM-149PT)Human breast ductal cancer cell Line (BT474)Human Pancreatic Carcinoma Cell Line (Capan2)
Respiratory System
Human Lung Cancer Cell Line (A549)Human Lung Cancer Cell Line (NCI-H1299)Human Lung Cancer Cell Line (NCI-H520)Human Lung Cancer Cell Line (SK-MES-1)Mouse Lewis lung carcinoma Cell Line (LLC)Human Bronchial Epithelial Cell Line (BEAS-2B)Human Lung Cancer Cell Line (HCC827)Porcine alveolar macrophage cell line (3D4/21)Human lung adenocarcinoma Cell Line (PC9)Human Lung Cancer Cell Line (NCI-H1650)Human Lung Cancer Cell Line (NCI-H727)Human lung adenocarcinoma Cell Line (NCI-H1975)Human lung cancer Cell Line (NCI-H292)Human Lung Cancer Cell Line (NCI-H23)Human Lung Cancer Cell Line (MSTO-211H)Human Lung Cancer Cell Line (NCI-H358)Human Non-small Cell Lung Carcinoma Cell Line (NCI-H460)Human lung adenocarcinoma Cell Line (Calu-3)Human Fetal Lung Fibroblast Cell Line (MRC-5)Human Highly Metastatic Lung Cancer Cell Line (95-D)Human Colon Carcinoma Cell Line (T84)MLE-12 Cell Complete Medium (MLE-12)Human Fetal Lung Fibroblast Cell Line (WI-38)Human Lung Cancer Cell Line (DMS114))Human lung adenocarcinoma Cell Line (NCI-H1373)Human Lung Cancer Cell Line (NCI-H1703)Human Lung Cancer Cell Line (DMS 53)Human Small Cell Lung Cancer Cell Line (NCI-H82 [H82])Human Small Cell Lung Cancer Cell Line (SHP-77)Human Lung Cancer Cell Line (NCI-H2170)
Reproductive System
Human Amniotic Cell Line (WISH)Human Highly Metastatic Ovarian Cancer Cell Line (HO-8910PM)African green monkey kidney Cell Line (Marc-145)Human Cervical Carcinoma Cell Line (Hela)Human ovarian cancer Cell Line (OVCAR-3)Human Ovarian Adenocarcinoma Cell Line (Caov-3)Mouse Embryonic Fibroblasts (NIH/3T3)Mouse Fibroblast Cell Line (TM3)Human Villous Trophoblast (HTR-8/SVneo)Human Ovarian Granulosa Cell Line (KGN)Chinese Hamster Ovary Cell Line (CHO-K1)Human Endometrial Adenocarcinoma Cell Line (MFE-280)Mouse ovarian epithelial cancer Cell (ID8)Human endometrial adenocarcinoma Cell (HEC-1-B)Human Endometrial Carcinoma Cell Line (ISHIKAWA)Human Cervical Carcinoma Cell Line (C-33 A)Human Cervical Carcinoma Cell Line (Ca Ski)Human Endometrial Carcinoma Cell Line (KLE)Mouse Teratocarcinoma Cell Line (P19)Human Ovarian Clear Cell Carcinoma Cell Line (ES-2)Human Endometrial Adenocarcinoma Cell Line (AN3 CA)Human Cervical Carcinoma Cell Line (hela 229)Human Ovarian Adenocarcinoma Cell Line (SK-OV-3)Human Choriocarcinoma Cell Line (Bewo)
Circulatory System
Rat Cardiac Myocytes (H9c2(2-1))Mouse Myoblast Cell Line (C2C12)Human Cardiac Myocytes (AC16)Mouse Aortic Smooth Muscle Cell Line (MOVAS)
Blood and lymphatic System
Mouse Hybridoma Cell Line (AE-1)Hybridoma (Anti-Cd3) Cell Line (OKT 3)Human Monocytic Cell Line (THP-1)Human Histiocytic Lymphoma Cell Line (U-937)Human T Lymphocyte Cell Line (Jurkat, Clone E6-1)Human Acute Lymphocyte Cell Line (OCI-Aml-3)Human myelogenous Leukemia Cell Line (K-562)Mouse Macrophage Cell Line (RAW 264.7)Human Acute Promyelocytic Leukemia Cell Line (HL-60)Human B Lymphoma Cell Line (U-2932)Mouse B Lymphoma Cell Line (A20)Human Myelomonocytic Leukemia Cell Line (MV4-11)Human B Lymphoma Cell Line (SU-DHL-4)Human Acute Myeloid Leukemia Cell Line (KG-1a)Human Erythroleukemia Cell Line (HEL)Mouse Pro B Cell Line (BAF3)Human acute promyelocytic leukemia cell line (NB4)Human T-lymphocyte Cell Line (Hut-78)Human B Lymphocytes Cell Line (GM12878)Mouse Lymphoma Cell Line (EL4)Mouse Macrophage Cell Line (Ana-1)Human Erythroleukemia Cell Line (TF-1)Human Lymphoma Cell Line (BC-3)Human Multiple Myeloma Peripheral Blood B Lymphocytes (RPMI 8226)Human Burkitt's Lymphoma B Lymphocytes (Ramos)Human B lymphoma cell line (SU-DHL-6)Human Acute Lymphoblastic Leukemia Cell Line (Non-B Non-T)(Reh)Human Acute Lymphocyte Cell Line (Kasumi-1)Human Mantle Cell Lymphoma Cell Line (JeKo-1)Human peripheral blood mantle lymphoma Cell Line (Mino)Human Acute Myeloid Leukemia Cell Line (MOLM13)Human B lymphocytic leukaemia Cell Line (NALM-6)Human B lymphoma Cell Line (SU-DHL-8)
Brain and Nervous System
Rat Glioblastoma Cell Line (C6)Mouse Hippocampal Neuronal Cell Line (HT-22)Human Neuroblastoma Cell Line (SK-N-SH)Human Neuroblastoma Cell Line (SH-SY5Y)Human glioblastoma Cell Line (U-87 MG)Human glioblastoma Cell Line (U251MG)Mouse Anterior Parietal Bone Cell Line (MC3T3-E1 Subclone 14)Mouse microglia Cell Line (BV2)Human microglial clone 3 Cell Line (HMC3)Mouse neuroblastoma Cell Line (Neuro-2a)Human glioblastoma Cell Line (LN-229)Mouse Glioblastoma Cell Line (GL261)Human Glioblastoma Cell Line (T98G)Human Glioblastoma Cell Line (A-172)Human Neuroblastoma Cell Line (SK-N-BE(2))Human Brain Astrocytoma Cell Line (Hs 683)Rat Glioblastoma Cell Line (RG2)
Urinary System
African green monkey kidney Cell (Vero)African green monkey kidney Cell (VERO C1008 (E6))Dog Kidney Cell Line (MDCK(NBL-2))Human Embryonic Kidney Cell Line (293T)Human Embryonic Kidney Cell Line (293T)Human Embryonic Kidney Cell Line (HEK293)Rat adrenal pheochromocytoma Cell Line (Low differentiation)(PC-12)Human Bladder Transitional Cell Carcinoma Cell Line (T24)Human bladder carcinoma cell line (5637)Mouse Bladder Cancer Cell (MB-49)Human renal cell carcinoma Cell Line (786-0)Human renal cell carcinoma Cell Line(MS751)Mouse podocyte Cell Line(MPC-5)Pig Kidney Cell line(PK-15)Human Embryonic Kidney Cell Line (2V6.11)Human Bladder Transitional Cell Carcinoma Cell Line (SW780)Transformed (SV40) African Green Monkey Kidney Cell Line (COS-1)Transformed (SV40) African Green Monkey Kidney Cell Line (COS-7)Rat Kidney Cell Line (NRK-52E)Mouse Renal Adenocarcinoma Cell Line (RenCa)Bovine Kidney (MDBK) Cell Line (MDBK (NBL-1))Hamster Kidney Fibroblasts (BHK-21)Human Renal Proximal Tubular Epithelial Cell Line (HK-2)Human Urinary Bladder Squamous Cell Carcinoma Cell Line (SCaBER)Mouse Renal Adenocarcinoma Cell Line (RAG)Mouse Bladder Cancer Cell (MBT-2)Human renal carcinoma Cell Line (OS-RC-2)
Skeleton, Articulus, Soft Tissue, Derma System
Human Umbilical Vein Cell Line (EA.hy926)Mouse Lewis lung carcinoma Cell Line (U-2 OS)Human Osteosarcoma Cell Line (Saos-2)Human skin squamous carcinoma Cell Line (A-431)Murine melanoma Cell Line (B16-F10)Human malignant melanoma Cell Line (A-375)Human Skin Fibroblast Cell Line (BJ)Mouse Fibroblast Cell Line (L-929)Mouse Lewis lung carcinoma Cell Line (SW 1353)Human fibrosarcoma Cell Line (HT-1080)Rat osteosarcoma Cell Line(UMR-106)Human Immortalized Keratinocytes Cell Line (HaCaT)Chicken Fibroblast Cell Line (DF1)Human Multiple Myeloma Cell Line (MM.1S)Human Corneal Epithelial Cell Line (HCE-T)(HCE-T)Immortalized Human Lens Epithelial Adherent Cell Line (SRA01/04)Mouse Lewis lung carcinoma Cell Line (MG-63)Mouse Osteosarcoma Cell Line (K7M2-WT)Mouse Lewis lung carcinoma Cell Line (143B)Mouse Subcutaneous Connective Tissue Cell Line (A9)Mouse squamous carcinoma Cell Line (SCC-7)Human Choriocarcinoma Cell Line (JAR)Human bone osteosarcoma epithelial Cell Line (KHOS-240S)Mouse Chondrogenic Cell Line (ATDC5)
Stem Cell Lines
Human induced pluripotent stem cells (iPSC) (IPSC-DYR0100)
Ocular, Otolaryngologic and Oral System
Human Pharyngeal Carcinoma Cell Line (Pleural Effusion Metastasis) (Detroit 562)Human retinal pigment epithelial Cell Line (ARPE-19)Human Nasopharyngeal Carcinoma Cell Line (CNE1)Human oral squamous carcinoma Cell Line (HSC3)Human nasopharyngeal carcinoma Cell Line (HK-1)Human oral squamous carcinoma cell line (CAL-27)Mouse Cochlear Hair Cell Line (HEI-OC1)Human Retinoblastoma Cell Line (Y79)Human Tongue Squamous Carcinoma Cell Line (SCC-9)Human Pharyngeal Squamous Cell Carcinoma Cell Line (FaDu)
Workflow
Plasmid with Selection Marker
Cell Transfection/ Transduction
Selection
Expansion
Clone Expansion & Single - cell Clone Isolation
Expression Detection
QC and Cryopreservation
Plasmid with Selection Marker
Cell Transfection/ Transduction
Selection
Expansion
Clone Expansion & Single - cell Clone Isolation
Expression Detection
QC and Cryopreservation
Representative publications
Overexpressing PRDX5 protein on LNCaP cell line
IF=15.1
Advanced Science
Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer
Jiangnan University
Abstract:
Recently, Dr. Rong Wang, from a research group led by Professor Yong Q Chen from Jiangnan University, China, published a research paper titled "Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer" on Advanced Science (IF=15.1). In this study, CRISPR-Cas9 PRDX5 knockout 22Rv1 cells and LNCaP cells with lentiviral overexpression of PRDX5 gene constructed by Ubigene were used to discover the new mechanism of PRDX5 affecting the progress of castration-resistant prostate cancer (CRPC) by regulating redox , and to explore the therapeutic effect of its targeting new small molecule drug Polaprezinc (POL) on CRPC in cells, animals and clinic. It provides a new target for clinical drug resistance of prostate cancer, and provides the possibility of new drugs combined with antiandrogen for patients with CRPC, so as to prolong the life cycle of patients with CRPC. View details
Recently, Dr. Rong Wang, from a research group led by Professor Yong Q Chen from Jiangnan University, China, published a research paper titled "Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer" on Advanced Science (IF=15.1). In this study, CRISPR-Cas9 PRDX5 knockout 22Rv1 cells and LNCaP cells with lentiviral overexpression of PRDX5 gene constructed by Ubigene were used to discover the new mechanism of PRDX5 affecting the progress of castration-resistant prostate cancer (CRPC) by regulating redox , and to explore the therapeutic effect of its targeting new small molecule drug Polaprezinc (POL) on CRPC in cells, animals and clinic. It provides a new target for clinical drug resistance of prostate cancer, and provides the possibility of new drugs combined with antiandrogen for patients with CRPC, so as to prolong the life cycle of patients with CRPC. View details
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HeLa and HepG2 cell lines overexpressing ACE2
IF=8.2
Acta Pharmacologica Sinica
SARS-CoV-2 receptor binding domain radio-probe: a non-invasive approach for angiotensin-converting enzyme 2 mapping in mice
Published by Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), NMPA Key Laboratory for Research and Evaluation of Radiopharmaceuticals (National Medical Products Administration), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, China
Abstract:
In order to verify the affinity of angiotensin-converting enzyme ACE at the cellular level, the author successfully constructed 124I-RBD as a novel targeted molecular probe for COVID-19 for the first time with the help of HeLa and HepG2 cell lines overexpressing ACE2 provided by Ubigene and confirmed that 124I-RBD antibody has high affinity and selectivity for ACE2 through the binding experiment of RBD and human ACE2 receptor. View details
In order to verify the affinity of angiotensin-converting enzyme ACE at the cellular level, the author successfully constructed 124I-RBD as a novel targeted molecular probe for COVID-19 for the first time with the help of HeLa and HepG2 cell lines overexpressing ACE2 provided by Ubigene and confirmed that 124I-RBD antibody has high affinity and selectivity for ACE2 through the binding experiment of RBD and human ACE2 receptor. View details
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MSCs overexpression Tandab
IF=7.5
Stem Cell Research & Therapy
Construction of tandem diabody (IL-6/CD20)-secreting human umbilical cord mesenchymal stem cells and its experimental treatment on diffuse large B cell lymphoma
Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China.
Abstract:
This paper uses the Tandab overexpression stable cell line constructed by Ubigene and empty control stable cell line to study the therapeutic effect of Tandab overexpressing MSCs on diffuse large B-cell lymphoma (DLBCL). The results showed that Tandab(IL-6/CD20) protein could target CD20-positive DLBCL cells, inhibit IL-6-related signaling pathway, and eliminate the promoting effect of IL-6 on lymphoma cells, which provided a potential strategy for the treatment of DLBCL. View details
This paper uses the Tandab overexpression stable cell line constructed by Ubigene and empty control stable cell line to study the therapeutic effect of Tandab overexpressing MSCs on diffuse large B-cell lymphoma (DLBCL). The results showed that Tandab(IL-6/CD20) protein could target CD20-positive DLBCL cells, inhibit IL-6-related signaling pathway, and eliminate the promoting effect of IL-6 on lymphoma cells, which provided a potential strategy for the treatment of DLBCL. View details
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CT26 cells overexpressing luciferase
IF=5.2
Cancers
Gas Flow Shaping via Novel Modular Nozzle System (MoNoS) Augments kINPen-Mediated Toxicity and Immunogenicity in Tumor Organoids
Published by Greifswald University Medical Center and the Leibniz Institute for Plasma Science and Technology in Germany
Abstract:
Gas plasma is a new tool for cancer treatment, among which the cold plasma jet kINPen has the most significant function. This study developed the MoNoS system to improve the flow properties of kiNPen using mouse CT26 cells overexpressing luciferase provided by Ubigene as research materials. Research has found that under clinically relevant raw gas flux, kINPen therapy adapted to MoNoS can significantly reduce cancer cell growth and increase tumor immunogenicity, while verifying its safety. This result further demonstrates the feasibility of using flow-shaping adapters (such as MoNoS) to enhance gas plasma jet therapy for cancer. View details
Gas plasma is a new tool for cancer treatment, among which the cold plasma jet kINPen has the most significant function. This study developed the MoNoS system to improve the flow properties of kiNPen using mouse CT26 cells overexpressing luciferase provided by Ubigene as research materials. Research has found that under clinically relevant raw gas flux, kINPen therapy adapted to MoNoS can significantly reduce cancer cell growth and increase tumor immunogenicity, while verifying its safety. This result further demonstrates the feasibility of using flow-shaping adapters (such as MoNoS) to enhance gas plasma jet therapy for cancer. View details
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