Overexpression cell line

The overexpression cell line are important tools for studying gene functions, disease mechanisms, and drug effects. Increasing the expression level of specific genes is used to achieve the function enhancement of genes. In addition, it can also be used in complementation experiments to verify the phenotypic recovery after gene knockout.

The overexpression vector is transferred into the cells through lentiviral transduction or nucleofection. As per the optimal antibiotic screening concentration explored in advance, the cells would be screened until the control cells die out to obtain a positive cell line with gene stable expression. The EZ-OE™ technology developed by Ubigene is different from the traditional lentiviral method and transposon method. It can not only achieve genome site-specific integration and single copy, but also has low cost and fast turnaround!