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Antibody as a powerful tool for diagnosis and research, its quality problem has been paid close attention by researchers worldwide. In the 2003 Human Protein Atlas (HPA) program, researchers validated more than 9000 antibodies from 29 vendors using WB and IHC, including monoclonal and polyclonal antibodies, and found that 49% were completely ineffective, while only 7% of the antibodies proved to be fully potent and possessed pre- and post-experimental consistency. According to statistics, before 2016, the annual loss of funding for research due to the use of lack-of-validated antibodies was approximately US $800 million in the world. The "antibody crisis" created by the cross reactivity versus variability of antibodies continues to present each year with substantial loss of funding for research.

WB, IHC, peptide arrays, and protein arrays, and so on are the commonly used methods for antibody validation, but they all are not perfect with more or less disadvantages, such as time-consuming operation, inability to validate in high throughput, difficulty in quantification, and difficulty in standardization, which means that one antibody may perform well in one validation method but fail completely in the other. So traditional validation methods alone do not guarantee specificity in this antibody, and many antibody companies are actively searching for new alternatives to demonstrate the quality of their antibodies to the consumer.

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"Golden" standard for antibody validation - Gene KO cells

Validation of antibody specificity by KO cells, by virtue of their unique strengths, has become the "Golden" standard for antibody validation. The table below is a comparison of technical advantages and disadvantages of KO cell models and siRNA knockdown models:

Validation methods Advantages Disadvantages
KO cell models (single-cell clones) KO on DNA level, loss of expression of target protein, "Golden" standard for antibody validation Optimal for antibody specificity validation, a perfect negative control Applicable for large-scale bulk antibody screening Some essential genes make it difficult to construct KO cell lines Relatively high cost, difficult to bulk production
(pool)KO cell models (cell pool) KO on DNA level, down-regulation of target protein expression, applicable for antibody validation Strong, convincing negative control for antibody specificity validation Applicable for large-scale bulk antibody screening Applicable for large-scale bulk antibody screening Some cell lethal, apoptotic genes make it difficult to construct KO cell lines Incomplete KO (polyclonal) Incomplete KO (polyclonal)
siRNA knockdown models Gene knockdown on RNA level, down-regulation of target protein expression, can be used for antibody validation Can be used for antibody specificity validation, traditional negative control Applicable for large-scale bulk antibody screening Some cell lethal, apoptotic genes make it difficult to construct knockdown cell lines Knockdown on RNA level, which means it is difficult to regulate the knockdown level of target protein expression Unstable siRNA efficiency, incomplete KO Non DNA level knockdown, less convincing
  • Near haploid cell "UBI-001" launched
  • Ubigene's lead strengths
  • As an enterprise focused on the field of gene-editing cells, Ubigene by virtue of the exclusive Red Cotton™ gene editing system and CRISPR-U™ patented technology of gene editing, has successfully developed UBI-001 near haploid cell line over 2 years, it enables us to stably batch provide high-quality KO cell lysates.

    Adherent cells, fast proliferation, easy-to-transfect, strong colony formation ability

    Near-haploid cell line, high KO effeciency, nearly 100%

With a team of experts with over 12 years of experience in gene-editing, Ubigene has so far successfully KO over 5000 genes in over 100 types of cell lines, and successfully established an exclusive KO Cell Bank and gRNA Plasmid Bank, covering 10 major research areas, 8 major signaling pathways, 40 diseases, and over 1300 genes. Currently, Ubigene's annual capacity of KO cell lysates is expected to be over ten thousand.

  • Red Cotton™ CRISPR Gene Editing Designer

    Red Cotton™ CRISPR Gene Editing Designer

    Ubigene's exclusive and intelligent data processing system - Red Cotton™ CRISPR gene-editing system enables to batch and automatically generate gene knockout strategies, which is the foundation for high-throughput production of KO cell lysates.

  • CRISPR-U™技术服务

    CRISPR-U™ Technology

    Ubigene exclusive developed CRISPR-U™ system, comparied with conventinal CRISPR/Cas9 technology, its cutting efficiency is 10-20 times higher, providing the technical guarantee for the production of high-quality KO cell lysates.

  • EZ-editor™产品

    EZ-editor™ Gene-editing Products

    Ubigene's EZ-editor™ series products and IT tools can greatly simplify the preparation procedure of KO cell lysates, also further improve the cell transfection efficiency and single-cell clone formation rate, effectively scaling down the turnaround of services and production by at least 25%.

KO cell lysate products and services

KO cell lysates (clone)

More than 600 types of KO cell lysates (monoclonal) are now available in Ubigene, as well as custom KO cell lysate services >>

Product & Service Turnaround Quantity Price
KO cell lysates (clone) In-stock 1 week 150ug/vial/gene Starting from 320 USD
Custom service(UBI-001) 6-8 weeks 150ug/vial/gene Please Inquire >>

KO cell lysates (pool)

Custom KO cell lysates (pool) service is now available in Ubigene, cutting effect verified by polyclonal sequencing and KO effect guaranteed >>

Product & Service Turnaround Quantity Price
KO cell lysates (pool) Custom service(UBI-001) 4-6 weeks 150ug/vial/gene Please Inquire >>

EZ-editor™ Series Products

EZ-editor™ Series Products are the gene-editing innovation products innovatively developed by Ubigene, including 6 major product series of cell lines, culture medium, kits, gRNA plasmids, lentiviruses, KO cell lysates, comprehensively covering the experimental flow for gene-editing. Our products enable simplification of experimental flow and significant improvement of gene-editing efficiency. And the EZ-editor™ KO cell lysates will be the key words for a boom in antibody validation in the future!