PPIF Knockout cell line(Caco-2)

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PPIF Knockout cell line(Caco-2) Order

PPIF Knockout cell line(Caco-2)
Catalog#
YKO-H342
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PPIF Knockout cell line(Caco-2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PPIF Knockout cell line (Caco-2)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
PPIF
Gene ID
10105
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
77%MEM+20%FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
Target region
Knockout region: Exon 1 (based on transcript PPIF-201)
Validation: PCR+Sanger Sequencing
PPIF Knockout cell line(Caco-2) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Caco-2
STR Info (Cell bank)
Cell Line:CACO-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 1114111413.0
D7S820 11121112
D16S539 12121213
VWA 16181618
TH01 6666
AMEL XXXX
TPOX 911911
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PPIF gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Caco-2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PPIF gene knockout PPIF Knockout cell line (Caco-2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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