CCK8 assay will be used to detect the killing effect of the target drugs on the library cells in order to select the appropriate drug concentration for screening. Avoid that too low drug concentration has no killing effect on cells, or too high drug concentration causes all cells to be killed, so that cells cannot be enriched for NGS sequencing.
Analysis Of Screening Results By Different Algorithms According To Different Selection Methods
Cell Passaging And Expansion - To screen growth
essential genes (MLE Algorithm)
Drug Selection - To screen drug targets (RRA Algorithm)
The correlation Heatmap is used to show the correlation between samples and visualize the correlation coefficient between two samples.

Good Repeatability Of Screening Results

High Reliability
Sample Correlation Analysis - Sample Clustering Plot