H9c2(2-1)-CAS9
Red cotton OmniCell bank
downloadOverview
The Cas9 nuclease has been stably integrated into the host cell genome via genetic engineering methods, enabling stable, long-term expression of Cas9 protein. Cas9 stable cell lines serve as essential tools for CRISPR gene knockout, CRISPR library screening, and functional genomics studies. Ubigene has developed more than 500 Cas9 stable cell lines covering commonly used cell types. Gene knockout can be rapidly achieved by simply transfecting gRNA. Co-transfection of gRNA with donor DNA further enables gene knock-in or precise point mutations.
The Ubigene RKO-Cas9 cell line was generated using a lentiviral method and selected with antibiotics to achieve stable, high-level Cas9 expression. The RKO-CAS9 cell line has undergone functional editing validation, with results demonstrating that introduction of target gene gRNA can produce efficient DNA cleavage and indels, making it suitable for gene knockout, gRNA efficiency validation, or high-throughput CRISPR library screening in RKO cells.
Detailed Product Information
H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle.
Product Advantages
Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Plasmid Backbone Map
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Quality Control
| Sample Name | Target Name | Cт Mean | ΔCт |
|---|---|---|---|
| H9c2(2-1)-CAS9 | Cas9 | 18.64950752 | -0.14704705 |
| H9c2(2-1)-CAS9 | β-actin | 18.79655457 | |
| H9c2(2-1) | Cas9 | 33.91866684 | 15.06260490 |
| H9c2(2-1) | β-actin | 18.85606194 |
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Figure1.Cas9 stable expression cell line cutting efficiency validation
1. This cell line stably expresses Cas9 nuclease. Gene knockout can be achieved by transfecting gRNA, while gene knock-in/point mutations can be achieved by transfecting gRNA together with donor DNA.
2. The transfected gRNA can be either plasmid-based or synthetic/in vitro transcribed sgRNA. Transfection methods can include transient transfection (e.g., lipofection, electroporation) or stable transfection (e.g., lentiviral method).
3. Prolonged in vitro culture may result in genomic alterations in some cells, potentially leading to reduced Cas9 expression over time.
Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
Unpacking and Storage Instructions
Handling procedure
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