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293T-EGFP

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293T-EGFP
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Catalog#
YC-A007-EGFP-P
Size
1*10^6 Cells/vial (frozen vial)
Culture method
90% DMEM+10%FBS
Instruction
STR Report

Overview

293T-EGFP is a cell line stably expressing EGFP protein. This cell line was generated using lentivirus method and enables stable and high-efficiency expression of enhanced green fluorescent protein (EGFP). Unlike enzymatic reporter systems, no additional enzymes or substrates are required for signal detection. The cells emit strong fluorescence under UV or blue light excitation without significant quenching and can be detected via in vitro and in vivo bioluminescence assays.

293T-EGFP cell line has been optimized to maintain low passage number, high viability, and good condition. It is suitable for a wide range of applications, including high-throughput drug screening, in vivo fluorescence tracing, stable cell line construction, and serves as a control cell line in lentiviral infection experiments.

EGFP luminescence principle
EGFP luminescence principle
EGFP luminescence principle

Detailed Product Information

293T-EGFP
Product name
293T-EGFP
Reporter gene
EGFP
Catalog number
YC-A007-EGFP-P
Resistance gene
Puromycin
Product category
EGFP Stable Cell Lines
Mycoplasma test
Negative
Species
Human
Accession
-
Cell morphology
Epithelial-like, adherent
Shipping condition
Frozen vials; dry-ice package
Passage ratio
1:3~1:6
Storage condition
Liquid nitrogen
Culture method
90% DMEM+10%FBS
Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Background

The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen.

Product Advantages

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Plasmid Backbone Map

Plasmid Backbone Map

View Picture

Quality Control

1. Bacteria and fungi test
None Detected.
2. Mycoplasma test
Negative.
3. Phenotypic stability
Expression stability has been confirmed across multiple cell passages.
4. STR Authentication

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

References

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