A2M Knockout cell line (Jurkat, Clone E6-1) – Genotype Verified & Ready-to-use

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A2M Knockout cell line (Jurkat, Clone E6-1)

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Catalog#

YKO-HB50177

Species

Human

Target Cell Line

Jurkat, Clone E6-1

Size

1*10^6

Gene

A2M

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

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Overview

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

The Jurkat, Clone E6-1 cell line was established from the peripheral blood of a 14-year-old male patient and is a derivative of the Jurkat T-lymphocytic cell line. This clone was generated from Jurkat E6-1 cells through gamma irradiation and complement-mediated selection using anti-CD45 antibodies. It exhibits markedly reduced CD45 expression (5–8% of the parental line), resulting in impaired signal transduction through the T-cell receptor (TcR) complex and the accessory molecule CD2. The cells can produce interleukin-2 (IL-2) following stimulation with calcium ionophore and phorbol ester but show defective IL-2 production after TcR or CD2 activation. This cell line is used as a model for studying T-cell signaling pathways and immune regulation.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the A2M gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into Jurkat, Clone E6-1 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, A2M Knockout cell line (Jurkat, Clone E6-1) is expanded and cryopreserved for downstream applications.

Product Overview

A2M Knockout cell line (Jurkat, Clone E6-1)

The A2M Knockout cell line (Jurkat, Clone E6-1) is generated by targeted disruption of the A2M gene in Jurkat, Clone E6-1 cells. This cell line is designed to investigate the role of A2M in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

A2M Knockout cell line (Jurkat, Clone E6-1)

Gene ID

2

Catalog

YKO-HB50177

Gene Name

A2M

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Human

Mycoplasma test

Negative

Cell Morphology

-

Catalog Status

-

Culture method

-

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

-

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. Knockout Strategy

Targeted knockout region: EExon 7

Reference Transcript: NM_001347423

Frameshift Mutation Strategy

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?