AAAS Knockout cell line (Caco-2)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

AAAS (aladin WD repeat nucleoporin) encodes a WD-repeat protein that is a component of the nuclear pore complex. The protein is anchored within the nuclear pore and may contribute to normal development and function of the peripheral and central nervous systems. AAAS therefore participates in nuclear pore complex organization and cellular nucleocytoplasmic transport processes.

Caco-2 is a human colorectal adenocarcinoma cell line established from a primary colon tumor of a 72-year-old male patient. The cells can form differentiated intestinal epithelial-like monolayers with characteristics including microvilli, tight junctions, dome formation, and transport functionality. Caco-2 cells also produce mucus and can form moderately differentiated adenocarcinoma-like tumors in nude mice. The cell line is widely used as an in vitro model for studying intestinal epithelial differentiation, barrier function, permeability, drug absorption, and colorectal cancer biology.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.