ABHD16A Knockout cell line (HCT-15)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ABHD16A (abhydrolase domain containing 16A, phospholipase) encodes a phospholipase associated with cellular immune functions. The protein belongs to a group of genes located within the major histocompatibility complex class III region and is thought to participate in aspects of immune regulation. ABHD16A is therefore relevant to studies investigating phospholipid metabolism and immune-related cellular processes.

HCT-15 is a human colorectal adenocarcinoma cell line established from a colorectal tumor. The cells are tumorigenic in nude mice and share a common genetic origin with several colorectal cancer cell lines, including DLD-1, HCT-8, and HRT-18, as confirmed by STR profiling. HCT-15 is widely used as a model for studying colorectal cancer biology, tumorigenesis, genetic alterations, and anticancer drug responses.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.