AKAP1 Knockout cell line （U-87 MG）

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

AKAP1 (A-kinase anchoring protein 1)encodes a member of the A-kinase anchoring protein family that localizes protein kinase A (PKA) to the mitochondrial outer membrane. By spatially organizing PKA signaling, AKAP1 regulates cAMP-dependent signal transduction and may contribute to mitochondrial function and intracellular RNA localization. It is widely studied in compartmentalized signaling and mitochondrial biology.

The U-87 MG cell line was derived from a malignant glioma of a female patient using an explant technique by J. Ponten and colleagues between 1966 and 1969. This human glioblastoma model has been extensively characterized and is reported to form malignant tumors consistent with glioblastoma in nude mice. U-87 MG is widely used in neuro-oncology research as an in vitro model for studying glioma biology, tumor growth mechanisms, cancer cell signaling, and evaluation of therapeutic strategies for malignant brain tumors.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.