ANO1 Knockout cell line (PC-3)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ANO1 (anoctamin 1) encodes a membrane protein with ligand-gated monoatomic ion channel and iodide transmembrane transporter activities. It participates in anion transport, mucus secretion, and glucose-stimulated insulin secretion. ANO1 is localized to the apical plasma membrane and nucleoplasm and contributes to ion transport and secretory processes in epithelial and other cell types.

PC-3 is a human prostate cancer cell line established from a bone marrow metastasis of a poorly differentiated grade IV prostatic adenocarcinoma from a 62-year-old male patient after androgen suppression therapy. The cells are androgen-independent, exhibit low acid phosphatase and testosterone-5-alpha reductase activity, and are capable of growing in soft agar and forming tumors in nude mice. PC-3 is widely used as a model for studying advanced prostate cancer biology, androgen-independent tumor progression, metastasis, and anticancer drug development.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.