ARID1A Knockout cell line (HEY) – Genotype Verified & Ready-to-use

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ARID1A Knockout cell line (HEY)

Copy Product Info

Catalog#

YKO-H2378

Species

Human

Target Cell Line

HEY

Size

1*10^6

Gene

ARID1A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

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Overview

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ARID1A (AT-rich interaction domain 1A) encodes a subunit of the SWI/SNF family of ATP-dependent chromatin remodeling complexes. ARID1A contributes to regulation of gene transcription by altering chromatin structure and accessibility and may help confer target specificity to the remodeling complex through DNA and protein interactions. It therefore plays an important role in chromatin-mediated control of gene expression.

Human high-grade ovarian serous adenocarcinoma cells derived in situ from the ovary are represented by HEY. The female-derived line is microsatellite stable and carries homozygous BRAF p.Gly464Glu and heterozygous KRAS p.Gly12Asp mutations.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the ARID1A gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into HEY cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, ARID1A Knockout cell line (HEY) is expanded and cryopreserved for downstream applications.

Product Overview

ARID1A Knockout cell line (HEY)

The ARID1A Knockout cell line (HEY) is generated by targeted disruption of the ARID1A gene in HEY cells. This cell line is designed to investigate the role of ARID1A in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

ARID1A Knockout cell line (HEY)

Gene ID

8289

Catalog

YKO-H2378

Gene Name

ARID1A

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Human

Mycoplasma test

Negative

Cell Morphology

Epithelial-like, adheren

Catalog Status

-

Culture method

90%DMEM+10%FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1:2~1:3

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. STR Authentication

Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?