BACH1 Knockout cell line （SiHa）

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

BACH1 (BTB domain and CNC homolog 1) encodes a CNC-bZIP transcription factor containing BTB/POZ domains that facilitate protein-protein interactions and oligomerization. BACH1 forms heterodimers with MafK and can repress transcription from Maf recognition elements. Through DNA binding and recruitment of transcriptional regulatory complexes, BACH1 regulates gene expression programs responsive to cellular and environmental signals. Its transcriptional activity is relevant to processes including oxidative stress responses, cellular differentiation, and regulation of metabolic and inflammatory pathways.

Human cervical squamous carcinoma cells containing an integrated HPV16 genome are represented by Siha. The line was established from the cervix of a 55-year-old woman and is classified as an HPV16-related cervical squamous cell carcinoma. Its HPV16 genome contains a disruption of the E2 region and a small deletion, while the cells exhibit microsatellite stability.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.