CHLSN Knockout cell line (HCT 116) – Genotype Verified & Ready-to-use

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CHLSN Knockout cell line (HCT 116)

Copy Product Info

Catalog#

YKO-XN97086

Species

Human

Target Cell Line

HCT 116

Size

1*10^6

Gene

CHLSN

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Overview

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

CHP2 (calcineurin like EF-hand protein 2) encodes a small calcium-binding protein that regulates plasma membrane Na+/H+ exchange and contributes to control of intracellular pH. CHP2 can associate with and stimulate the phosphatase activity of calcineurin A, supporting a role in the calcineurin/NFAT signaling pathway. Its calcium-dependent regulatory functions connect ion homeostasis with intracellular signal transduction and may contribute to cellular responses involving calcineurin activity.

HCT 116 is a human colorectal carcinoma cell line established from a primary colon tumor of an adult male patient. The cells are tumorigenic in nude mice, form colonies in agarose, and carry a mutation in the RAS gene at codon 13. HCT 116 is widely used as a model for studying colorectal cancer biology, oncogenic signaling pathways, tumorigenesis, and anticancer drug responses.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the CHLSN gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into HCT 116 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, CHLSN Knockout cell line (HCT 116) is expanded and cryopreserved for downstream applications.

Product Overview

CHLSN Knockout cell line (HCT 116)

The CHLSN Knockout cell line (HCT 116) is generated by targeted disruption of the CHLSN gene in HCT 116 cells. This cell line is designed to investigate the role of CHLSN in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

CHLSN Knockout cell line (HCT 116)

Gene ID

84310

Catalog

YKO-XN97086

Gene Name

CHLSN

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Human

Mycoplasma test

Negative

Cell Morphology

Epithelial-like, adherent

Catalog Status

-

Culture method

90%McCOY's 5A+10% FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1:2~1:4

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. STR Authentication

Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?