COL12A1 Knockout cell line (AC16)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

COL12A1 (collagen type XII alpha 1 chain) encodes the alpha chain of type XII collagen, a member of the fibril-associated collagens with interrupted triple helices family. Type XII collagen forms homotrimers that associate with type I collagen fibrils and is thought to modify interactions between collagen fibers and the surrounding extracellular matrix. Through these matrix interactions, COL12A1 contributes to organization of connective tissue structure and regulation of extracellular matrix architecture.

Human cardiac ventricular cells are represented by AC16, an immortalized cell line derived from primary human ventricular tissue. The cells were generated by fusion of primary adult ventricular cardiomyocytes with a human fibroblast-like cell line carrying a temperature-sensitive SV40 large T antigen. AC16 exhibits cardiac-related characteristics and is used as an in vitro model for studies of human cardiomyocyte biology.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.