COPS9 Knockout cell line (HEK293)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

COPS9 (COP9 signalosome subunit 9) encodes a component of the COP9 signalosome involved in regulation of protein neddylation. COPS9 participates in cellular responses to ultraviolet stress and contributes to regulation of cell population proliferation. Through its role in the COP9 signalosome, the protein helps control post-translational modification of cellular proteins and associated regulatory processes.

The HEK293 cell line is a human embryonic kidney cell line derived from adenovirus 5-transformed human embryonic kidney cells. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions. HEK293 cells have been adapted into various derivatives, including suspension-adapted variants and engineered reporter lines for specific applications. This cell line is widely used in molecular biology, viral vector production, recombinant protein expression, gene expression studies, and research involving cellular signaling and genome engineering.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.