Egfr Knockout cell line (MC3T3-E1 Subclone 14)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

Epha2 (Eph receptor A2) encodes a receptor tyrosine kinase in the Eph family, which mediates cell-to-cell signaling involved in tissue organization and development. EPHA2 is associated with processes including blood vessel morphogenesis, nervous system development, lens fiber cell morphogenesis, mammary gland development, and osteoclast differentiation. The protein localizes to the cell surface and is expressed across multiple tissues. Mutations in its human ortholog are associated with several forms of cataract.

The MC3T3-E1 cell line is a foundational mouse osteoblast model derived from the cranial calvaria of newborn C57BL/6 mice. As referenced in image_2ede08.png, a series of subclones were isolated from the cloned but phenotypically heterogeneous parental MC3T3-E1 line to select for varying degrees of osteoblast differentiation and mineralization potential. This adherent line serves as a gold-standard in vitro platform for studying bone morphogenesis, osteogenesis pathway kinetics, and transcriptional regulation during matrix calcification.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.