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Enpp1 Knockout cell line (UMR-106)

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Enpp1 Knockout cell line (UMR-106) WB 1490
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Catalog#
YKO-R040
Species
Rat
Target Cell Line
UMR-106
Size
1*10^6
Gene
Enpp1
Storage conditions
Shipped on dry ice; Store in liquid nitrogen

Overview

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

Entpd1 (ectonucleoside triphosphate diphosphohydrolase 1) encodes an ectonucleotidase with ADP phosphatase and ribonucleoside triphosphate phosphatase activities. It contributes to nucleotide catabolism, G protein-coupled receptor signaling, and platelet activation. The protein is active on the external surface of the plasma membrane. Its human ortholog, ENTPD1, has been implicated in hereditary spastic paraplegia 64.

UMR-106 is a rat osteosarcoma cell line derived as a clonal derivative of a transplantable osteogenic sarcoma induced by radiophosphorus (³²P). The cells retain characteristics of the original tumor, including responsiveness to parathyroid hormone (PTH) and prostaglandins. UMR-106 is used as a model for studying bone tumor biology, osteoblast-related signaling, and skeletal regulation mechanisms.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.

KO Cell Construction Strategy
Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the Enpp1 gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into UMR-106 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, Enpp1 Knockout cell line (UMR-106) is expanded and cryopreserved for downstream applications.
KO Cell Construction Strategy

Product Overview

Enpp1 Knockout cell line (UMR-106)
The Enpp1 Knockout cell line (UMR-106) is generated by targeted disruption of the Enpp1 gene in UMR-106 cells. This cell line is designed to investigate the role of Enpp1 in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.
KO Cell Line
Enpp1 Knockout cell line (UMR-106)
Gene ID
85496
Catalog
YKO-R040
Gene Name
Enpp1
Product Category
Knockout(KO) Cell Line
Gene Information
NCBI
Species
Rat
Mycoplasma test
Negative
Cell Morphology
Epithelial-like, adherent
Catalog Status
-
Culture method
90%DMEM+10%FBS
Shipping
Cryopreserved Cells shipped in dry ice
Passage ratio
1：2~1：4
Storage Condition
Liquid nitrogen
Quality Control
All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.
Applications
For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test
None Detected.
2. Mycoplasma test
Negative.
3. Knockout Strategy

Targeted knockout region: E1

Reference Transcript: Enpp1-201

Frameshift Mutation Strategy

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?
If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:
(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.
(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.
Q2. What Information Is Required When Reporting KO Cell Product Issues?
Q3. How Should the KO cell Product Be Stored Upon Receipt?
Q4. Shipping Method
Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?

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