FBXL3 Knockout cell line (BEAS-2B)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

FBXL3 (F-box and leucine rich repeat protein 3) encodes an F-box protein containing leucine-rich repeats and functions as a component of SCF ubiquitin ligase complexes. F-box proteins contribute to substrate recognition within these complexes, which mediate phosphorylation-dependent ubiquitination. FBXL3 localizes to the nucleus and is relevant to studies of ubiquitin-dependent protein turnover, substrate recognition, and regulation of cellular protein stability.

BEAS-2B is an immortalized human bronchial epithelial cell line established from normal bronchial epithelium obtained from non-cancerous individuals. The cells were immortalized using a replication-defective SV40/adenovirus 12 hybrid (Ad12SV40) and cloned. BEAS-2B cells express epithelial markers including keratins and SV40 T antigen and can undergo squamous differentiation in response to serum exposure. The cell line is widely used as a model for studying normal airway epithelial biology, respiratory infection mechanisms, epithelial differentiation, environmental toxicity, and carcinogenesis.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.