FBXO32 Knockout cell line （Huh-7）

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

FBXO32 (F-box protein 32) encodes an F-box protein that functions as a substrate-recognition component of SCF ubiquitin ligase complexes. Through phosphorylation-dependent ubiquitination of target proteins, FBXO32 contributes to regulation of protein stability and cellular protein turnover. FBXO32 is strongly expressed during muscle atrophy, and loss of the gene has been associated with resistance to muscle wasting in experimental models, supporting a role in regulation of skeletal muscle protein catabolism.

A human hepatocellular carcinoma cell line of Japanese origin, established from the liver tumor of a 57-year-old male patient. The cells are classified as an adult hepatocellular carcinoma line and have been extensively characterized for their molecular and genetic properties. The cell line exhibits a predominantly East Asian genomic ancestry profile and carries documented sequence variations affecting genes including KDR, POLD3, TERT, and TP53.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.